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Table 2 Three-way ANOVA results of plasma ACTH and serum corticosterone levels

From: Maternal separation and TNBS-induced gut inflammation synergistically alter the sexually differentiated stress response in rats

Effects

df

F

P-level

no-CRD ACTH

 Sex

1

14.488

0.0001***

 MS

1

1.462

0.232

 TNBS

1

3.422

0.07

 Sex × MS

1

4.721

0.034*

 Sex × TNBS

1

2.611

0.112

 MS × TNBS

1

8.541

0.005*

 Sex × MS × TNBS

1

3.333

0.074

 Residuals

51

  

no-CRD corticosteron

 Sex

1

130.7

0.0001***

 MS

1

0.112

0.739

 TNBS

1

2.366

0.13

 Sex × MS

1

1.501

0.226

 Sex × TNBS

1

0.322

0.573

 MS × TNBS

1

6.843

0.012*

 Sex × MS × TNBS

1

1.708

0.197

 Residuals

52

  

CRD ACTH

 Sex

1

1.672

0.201

 MS

1

0.194

0.661

 TNBS

1

0.198

0.659

 Sex × MS

1

1.833

0.182

 Sex × TNBS

1

0.214

0.646

 MS × TNBS

1

0.672

0.416

 Sex × MS × TNBS

1

8.418

0.006*

 Residuals

47

  

CRD corticosterone

 Sex

1

35.862

0.0001***

 MS

1

0.329

0.569

 TNBS

1

1.253

0.269

 Sex × MS

1

0.069

0.794

 Sex × TNBS

1

0.043

0.836

 MS × TNBS

1

0.43

0.515

 Sex × MS × TNBS

1

0.329

0.569

 Residuals

48

  
  1. Results of 3-way ANOVA of neuroendocrine data with or without colorectal distention (CRD) with sex, maternal separation (MS), and previous inflammation (TNBS) as 3 factors were shown. *P < 0.05, **P = 0.001, and ***P = 0.0001